... system, and by HPLC on a C18 reverse-phase column It was found to be at least 98.4% pure Fig Structureof SDTG (A) and time course of inactivation of recombinant GST I by SDTG at pH 6.5 and 25 ... 2,4,6-trinitrobenzenesulfonic acid, and the amount of covalently bound SDTG was determined by subtraction of the total number of carboxy and amino groups in the modified and unmodified enzyme The results of this experiment ... course of inactivation of wild-type GST I and mutant Phe51Ala by SDTG Wild-type (r) and mutant Phe51Ala (j) were incubated in the presence of 72.7 and 92 lM SDTG, respectively, at pH 6.5 and 25...
... patterns and processes at different levels of biological organization: from the life histories of individuals, to the structureand dynamics of populations and communities, to the fluxes and pools of ... measuring and understanding the roles of different kinds of organisms in the flux and storage of elements in ecosystems The total biomass per unit area, W, is simply the sum of the body mass of all ... abundances and turnover of populations, the allocation of resources among coexisting species, and the fluxes and pools of energy and materials in ecosystems These exchanges are direct consequences of...
... the structureand PDB code shown below each panel (A) Overlap of isoform 337 with the structureof the longer isoform CfAFP-501 using the main chain of residues Thr23fiAsn90 in isoform 337 and ... other and with proteins that have a similar fold Structureof sbwAFP and TmAFP The structureof sbwAFP has been determined by X-ray ˚ crystallography to 2.5 A and by NMR at both 30 °C and °C ... method and PDB code shown below each panel (A) Overlap of X-ray structure with °C NMR structure using the main chain of residues Ser12fiThr70 in the structure alignment (B) Overlap of the X-ray structure...
... longer-chain (and nonglobular) versions of the structureof the Bowman±Birk inhibitor in Fig The X-ray crystal structure pi2 reveals that the /,w angles of most of the residues of the Bowman±Birk ... form of b strand [18] The positive bands at » 1675 cm)1 in the amide I region of the ROA spectra of b- and j-casein, which originate mainly in the peptide C O stretch, are characteristic of disordered ... the theme of PPII structureand rheomorphism is explored by a comparative ROA study, supplemented with DSC, of caseins, synucleins and tau, together with several mutants of a-synuclein and tau...
... study of problem solving action and report: I) the collected of data on problem solving and talk about problem solving, 2) development of a process model of these behaviors, and 3) use of coding ... problem solving from a record of the problem solving report and a record of moves made Then, we use this extracted trace to evaluate our model of the role of point of view in problem solving SUMMARY ... organization of the problem ("point of view"), and systematic multl-utterance structures used to express the forms of inference used to solve the problem ("Justificatlon argument structures")...
... CHAPTER – THE STRUCTUREAND DEVELOPMENT OF THE CEREAL PLANT 24 THE WHEAT BOOK THE WHEAT BOOK CHAPTER – THE STRUCTUREAND DEVELOPMENT OF THE CEREAL PLANT THE STRUCTUREAND DEVELOPMENT OF THE CEREAL ... andstructureof the plant evolves as the integration of many consecutive and interacting processes 25 CHAPTER – THE STRUCTUREAND DEVELOPMENT OF THE CEREAL PLANT THE WHEAT BOOK DESCRIPTION OF ... formation of florets within each spikelet and, later, the regression and death of some florets and spikelets and the death of some tillers This phase is also the time of greatest dry mass increase and...
... comprising mainly the b-strands The loops between strands B and C, known as the ‘hypervariable loop‘, D and E, E and F, F and G, and G and H are particularly tolerant of longer insertions [19] ... 300 and 315 K at pH7.0 Fig S12 Overlay of HSQC spectra collected at 300 K of single CCP1 and CCP2 andof pair of modules at neutral and acidic pH Doc S1 Intermodule cooperativity in the structure ... with aspartate and ⁄ or glutamate residues In the light of atomic structures of CCP1 and CCP2 (2qy0 [36] and 1gpz [37]), this perturbation might indicate that in CCP1, the side chain of H335 is fully...
... the effect of a mutation in this part of the molecule on the structureand properties of human sHsp Oligomeric structureof HSP22 and its mutants All samples of recombinant HSP22 and its mutants ... importance of this part of molecule of the sHsp is supported by the fact that mutations in the loop connecting the b5 and b7 strands, or in the b7 strand of sHsp, often correlate with the development of ... domain of human sHsp lacks the b6 strand detected in the structureof Hsp16.5 of M jannaschii and wheat Hsp16.9 and the loop connecting b5 and b7 is much shorter than the corresponding loop of bacterial...
... Annealing on the Crystal Structureand Surface Morphology To understand the effect of annealing on the structureand surface morphology of the magnesium borate hydroxide nanostructures, the as-prepared ... 5:149–157 of nanowhiskers with reduced size and uneven shapes The surfactants of various ionic phases (anionic, cationic and non-ionic) have been extensively used in the synthesis of nanostructures ... is a closer view of the nanowhisker deformed in the presence of electron beam, which is confirmed by the random bulging and smearing of the nanowhisker surface The deformation of the nanowhiskers...
... chloroplast ultrastructure, and leaf characteristics of high- and low-light plants andof sun and shade leaves Photosynth Res 2, 115-141 light E.M (1986) Correlation of activity and amount of ribulose ... a lower rate of C0 uptake and an appressed/non-appressed membrane ratio of about In the present study, a tion was found between negative correla- N P and the ratio of the length of appressed ... 2A) and between the ratio of the length of appressed to non-appressed thylakoid membranes and photon fluence rate (Fig 2B) This suggests that, in the canopy, acclimation of the thylakoid structure...
... by endothelial cells that have a unique structureand function essential for hepatic and systemic homeostasis Much of our understanding of the biology of the LSECs has been generated in experiments ... IM and BS conceived of the study IM carried out most of the experimental work and the writing AW and DGL participated in the determination of fenestration parameters, statistical analysis and ... Pertoft H, Eggertsen G, Sundstrom C: Functional and morphological characterization of cultures of Kupffer cells and liver endothelial cells prepared by means of density separation in Percoll, and...
... infrastructure (RNA and proteins) The structureand function of this information-processing complex are of great interest in the study of both normal cellular functions (such as differentiation and ... the organization of these components The initial implication of this situation leads to the traditional concept of the unit of Page 20 of 29 D’Onofrio and An Theoretical Biology and Medical Modelling ... examination of the structureand function of computer hard drives and DNA Theoretical Biology and Medical Modelling 2010 7:3 Submit your next manuscript to BioMed Central and take full advantage of: ...
... understand and express our perceptions of the world and our consciousness It is the means of representing reality and known as the ideational function It consists of experiential meanings and logical ... the field of my study and take “The meaning andstructureof President Nguyen Minh Triet’s speech addressed on the occasion of the 1000 th anniversary of the foundation of Thang Long – Hanoi: ... language and deals with rules of correct usage rather than with meaning and context of the text, functional grammar centers on both written and spoken language and emphasize how linguistic structures...
... both perfection of part and adequacy of collection In this book the use of ``program'' is focused on the creation, execution, and study ofprograms written in a dialect of Lisp for execution on ... in Lisp programs, and the construction of language processors is rarely an impediment to the rate of growth and change of large Lisp systems Finally, it is this very simplicity of syntax and semantics ... places of accuracy can we represent? Is the range of integers the same as the range of real numbers? Above and beyond these questions, of course, lies a collection of issues concerning roundoff and...
... domains of Spir-2 and PTPN13 were generated by RT-PCR and inserted into pEGFP-C1 and pGEX-4T-2 The N- and C-terminal regions of the v-KIND KIND2 and the MAP2 CD were generated by PCR and cloned ... series of GST-fused deletions of the CD2-1 region (Fig 3E) and examined the interactions between bacterially expressed proteins of these subregions (Fig S1C) and 1654 Fig Of all members of the ... in dendrites of cultured hippocampal neurons Fig S4 Domain structureof MAP2 and the alignment of v-KIND-binding core (BD) region of MAP2 CD2 domain (702–744 aa) in human, mouse and Gallus This...
... reproducibility, which is a function of the sampling, diluting, and filling of the counting chamber, as well as the choice of the right type of counting chamber and range of cell concentration Counting ... adaptation of the cell metabolism to growth, such as the increase of the levels of enzymes and metabolites involved in cell division and carbon fixation Figure 2.3 Five growth phases of micro-algae ... successfully in rectangular outdoor concrete ponds of 10-40 tons of water volume and a water depth of 1.5-2 m 2.3.7 Culture of sessile micro-algae Farmers of abalone (Haliotis sp.) have developed special...
... Number of circulations = values of matrix K 2 2 2 8 8 2 2 8 8 1 8 8 1 8 88 1 8 8 8 Ordinal index of point XYZ 2 2 Values of matrix NB Fig 2.2.3a: Computation of the matrices NB and K All elements of ... selection of points (max 64) falling into the specified circle surrounding with a radius of 44600 units The radius and maximal number of selected points was set by the used software – if one or both of ... K i , j1 This modification of smoothing suppresses oscillations and exceeding of local extremes, if they occur (see paragraph 2.4.1 Smoothness of interpolation and oscillations) 2.2.8 Iteration...